Scanning Probe Microscopy of Biospecific Interaction

MSU & ASU collaborative work under the sponsorship of NATO Science Program (Linkage Grant #LST.CLG.975.161)

Escherichia coli in air

Contact mode 13 micron

E. coli in air

Acoustic mode 8 micron

E. coli in liquid

Contact mode 10 micron

E. coli in liquid

MAC mode. 7.5 micron

MP-RNA complex after formamid treatment

MP-RNA. 0.5 micron

MP-RNA complex 20:1

MP-RNA. 0.5 micron

MP-RNA complex 60:1

MP-RNA. 0.7 micron

RNA release from virus capsid

RNA. 10 micron

Olga Kiselyova
Anastassia Bolshakova
    Participants:

    Aims:

      1. The dynamics and mechanics of DNA as studied by AFM
      2. Study of RNA-protein interactions
      3. Study of antigen-antibody interactions

    Recent activity:

      The main direction of collaboration work during the 1999:
        - visualization of DNA-protein and RNA-protein complexes
        - biospecific interaction with bacterial cellular wall
      MSU group has supplied ASU partners with newly developed FemtoScan image analysys software.

    Joint seminars:

    Publications:

    1. Bolshakova A.V., Yaminsky I.V. Atomic force microscopy studies of bacterial cells // NIMC-EAPS International Conference "Nonlinear Dynamics in Polymer Science and Related Fields" (Desna, Moscow Region, Russia, 11-15 October, 1999), Book of brief reports, pp. 146-149.
    2. Bolshakova A.V., Kiselyova O.I., Filonov A.S., Frolova O.Yu., Lyubchenko Yu.L. and Yaminsky I.V. Comparative studies of bacteria with atomic force microscopy operating in different modes // Ultramicroscopy, 68 (1-2), 121-128 (2001).
    3. Dubrovin E.V., Gallyamov M.O., Yaminsky I.V. Micromechanics of nucleic acids // Physics of Low-Dimensional Structures, v. 5/6, pp. 7-12 (2002).
    4. Kiselyova O.I., Galyamov M.O., Nasikan N.S., Yaminsky I.V., Karpova O.V., Novikov V.K. Scanning probe microscopy of biomacromolecules: nucleic acids, proteins and their complexes in "Frontiers of Multifunctional Nanosystems" (Eds. Buzanaeva E.V., Scharff P. - Kluwer Academic Publishers, Dordrecht, 2002, 500 pages), pp. 321-330.
    5. Yaminsky I.V. Patterns in biopolymers and other biological systems as observed by scanning probe microscopy // in "Recent Advances in Scanning Probe Microscopy of Polymers, ed. by V.V. Tsukruk and N.D. Spenser, Macromolecular Symposia, Wiley-VCH, v. 167, pp. 63-72 (2001).
    6. Kiselyova O.I., Bolshakova A.V., Frolova O.Yu., Filonov A.S., Lyubchenko Yu.L., Yaminsky I.V. Comparative Studies of E.coli Bacteria by means of Atomic Force Microscope // Abstracts of papers presented at the EMBL Conference "Scanning Probe Microscopy, Cantilever Sensors and Nanostructures, Heidelberg, Germany, May 28-31, 2000 P.26.
    7. Limansky, A. P., Shlyakhtenko, L. S., Hauss, S. Henderson, E. and Lyubchenko, Y. L. (2002) Aminomodified probes for atomic force microscopy. Probe Microscopy, in press
    8. Luda S. Shlyakhtenko, Alexander A. Gall, Alexander Filonov, Zoran Cerovac, Alexander Lushnikov and Yuri L. Lyubchenko (2002) SILATRANE-BASED SURFACE CHEMISTRY FOR IMMOBILIZATION OF DNA, PROTEIN-DNA COMPLEXES AND OTHER BIOLOGICAL MATERIALS. Ultramicroscopy, in press.


    Final Report

Cactuses AZ view Cactus Cactus







Anastassia V. Bolshakova, ATC, 2000